Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Abcam Mammalian Cell Lysis Buffer 5X (ab179835)
Mammalian Cell Lysis Buffer 5X (ab179835)
The product is subject to the following: Abcam Restricted Use Statement
Boston Bioproducts Inc EDTA (0.5 M, pH 7.4) - 500ml
EDTA [Ethylenediaminetetraacetic acid] solution is commonly used in various molecular biology and protein biochemistry applications that require the chelation of divalent metal cations, such as calcium (Ca2+) and magnesium (Mg2+). It is not soluble in water unless pH is adjusted accordingly. EDTA is used extensively as an anticoagulant in blood samples as it chelates the calcium present, preventing coagulation and preserving blood cell morphology. It is also used in cell lysis buffers.
TRIBIOSCIENCE INC Inoue Transformation Buffer, 100ML
Inoue Transformation Buffer is used for the preparation of high-efficient competent cells. This product is based on the Inoue method for the preparation and transformation of E. Coli component cells.
Complement Technology Inc GVBE (GVBo with EDTA) 250ml
GVBE is an isotonic buffer used in complement assays to prevent complement activation or to stop ongoing complement activation when added at the end of an incubation
Cliawaived Inc Transport Tube, 3mL Phosphate Based Saline PBS (400 swabs and PBS Tubes per box)
Transport Tube, 3mL Phosphate Based Saline PBS. 102mm length vial contains 3ml of PBS solution. Includes mini-flocked swab with synthetic fibers and plastic shaft and break point at 80mm. INTENDED USEThe KaiBiLiTM Extended ViralTrans PBS is phosphate buffer saline which can maintain pH and osmotic balance, as well as provide cells with water and essential inorganic ions.SUMMARYAppropriate specimen collection and transport is important to accurate laboratory diagnosis of infectious disease. Not only personnel operating skills, but also a proper specimen collection and transport system are essential attributes for reliable diagnosis result. The KaiBiLiTM Extended ViralTrans PBS is suitable for collection, transport of clinical specimens.
Meso Scale Discovery MSD GOLD Read Buffer B | 200mL
MSD GOLD Read Buffer B, Read buffer is an essential component of MSD immunoassays: it is required for the generation of electrochemiluminescence (ECL) signals. MSD GOLD Read Buffer B was optimized for use in all MSD assays and can be used as a replacement for MSD GOLD Read Buffer A and MSD Read Buffer T (4x). MSD GOLD Read Buffer B is also recommended for assays that measure whole cell, vesicles, or other membrane-containing analytes. MSD GOLD Read Buffer B has stringent quality control release criteria that ensure lot-to-lot reproducibility. The specifications are designed to meet the requirements of critical assays and longitudinal studies.
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X) - 4L
The Tris Buffered Saline (TBS) is an isotonic solution frequently used in biological research due to its non-toxic nature. The formulation of TBS includes Tris (tris(hydroxymethyl)aminomethane), which serves as a buffering agent, and Sodium Chloride (NaCl) salt. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X) - 1L
The Tris Buffered Saline (TBS) is an isotonic solution frequently used in biological research due to its non-toxic nature. The formulation of TBS includes Tris (tris(hydroxymethyl)aminomethane), which serves as a buffering agent, and Sodium Chloride (NaCl) salt. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Boston Bioproducts Inc EDTA (0.5 M, pH 7.4, pH Adjusted with KOH) - 250ml
EDTA [Ethylenediaminetetraacetic acid] solution is commonly used in various molecular biology and protein biochemistry applications that require the chelation of divalent metal cations, such as calcium (Ca2+) and magnesium (Mg2+). EDTA is used extensively as an anticoagulant in blood samples as it chelates the calcium present, preventing coagulation and preserving blood cell morphology. It is also used in cell lysis buffers. This solution is adjusted with KOH.
Teknova 200mM Tris, pH 7.0. 1mL, Sterile. 50 Pack
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | 77-86-1 | MFCD00004679 | 100G
Tris(hydroxymethyl)aminomethane ACS reagent, >=99.8% | Purity: >=99.8%; 99.8-100.1% | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100G
Boston Bioproducts Inc Tris-Acetate-EDTA Buffer, TAE (50X) - 1L
The Tris-Acetate-EDTA (TAE) Buffer is a pH regulator and chelator often used in molecular biology techniques such as agarose gel electrophoresis. Supporting a pH range of 8.1-8.4, the TAE Buffer combines tris base, acetic acid, and EDTA (Ethylenediaminetetraacetic Acid) to form a solution efficient in separating nucleic acids as a running buffer, or as a component of agarose gels. This Buffer is prepared in a 50X stock solution, and can be diluted to ideal concentrations.